X-ray transparent microfluidic platforms for membrane protein crystallization with microseeds
نویسندگان
چکیده
منابع مشابه
X-ray transparent microfluidic chips for high-throughput screening and optimization of in meso membrane protein crystallization.
Elucidating and clarifying the function of membrane proteins ultimately requires atomic resolution structures as determined most commonly by X-ray crystallography. Many high impact membrane protein structures have resulted from advanced techniques such as in meso crystallization that present technical difficulties for the set-up and scale-out of high-throughput crystallization experiments. In p...
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X-ray transparent Microfluidics for Protein Crystallization and Biomineralization A dissertation presented to the Faculty of the Graduate School of Arts and Sciences of Brandeis University, Waltham, Massachusetts by Achini Opathalage Protein crystallization demands the fundamental understanding of nucleation and applying techniques to find the optimal conditions to achieve the kinetic pathway f...
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Crystallization from lipidic mesophase matrices is a promising route to diffraction-quality crystals and structures of membrane proteins. The microfluidic approach reported here eliminates two bottlenecks of the standard mesophase-based crystallization protocols: (i) manual preparation of viscous mesophases and (ii) manual harvesting of often small and fragile protein crystals. In the approach ...
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We describe a microfluidic approach to optimize crystallization of active pharmaceutical ingredients (APIs) and their solid forms (cocrystals) via crystal seeding. Subsequent on-chip X-ray diffraction is used to verify the crystal from. The microfluidic platform comprises an 8 × 9 well array that enables screening of seeding conditions (dilutions) by metering of API solution or API/cocrystal fo...
متن کاملProtein crystallization for X-ray crystallography.
Using the three-dimensional structure of biological macromolecules to infer how they function is one of the most important fields of modern biology. The availability of atomic resolution structures provides a deep and unique understanding of protein function, and helps to unravel the inner workings of the living cell. To date, 86% of the Protein Data Bank (rcsb-PDB) entries are macromolecular s...
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ژورنال
عنوان ژورنال: Lab on a Chip
سال: 2018
ISSN: 1473-0197,1473-0189
DOI: 10.1039/c7lc01141e